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目的探索人类急性髓系白血病（ＡＭＬ）逆转录病毒病因学的可能性。方法应用细胞培养，Ｄ－ＩＧＳＳ，逆转录酶测定，ＸＣ细胞合胞体试验以及电镜技术对白血病细胞和正常造血细胞进行逆转录病毒相关抗原（ＲＶＡＡｇ），逆转录酶活性，病毒致细胞病变效应（ＣＰＥ）及病毒颗粒进行检测与分析。结果３２例ＡＭＬ患者培养前后白血病细胞样本ＲＶＡＡｇ阳性检出率分别为５０．０％和８７．５％。其中１４例ＲＶＡＡｇ阳性样本同时表达逆转录酶活性且ＸＣ细胞合胞体试验阳性。电镜观察证实，７５％ＡＭＬ细胞样本中可见典型的Ｃ型逆转录病毒颗粒。２０例正常对照细胞培养前后样本的ＲＶＡＡｇ，逆转录酶活性，合胞体试验及电镜观察结果均为阴性。结论ＡＭＬ患者的白血病细胞中含有完整的逆转录病毒基因组。在体外经适当的诱导后能以完整病毒颗粒通过芽生方式释放到细胞外
Objective To explore the possibility of the etiology of human acute myeloid leukemia (AML) retrovirus. Methods Cell culture, D-IGSS, reverse transcriptase assay, XC cell syncytium and electron microscopy were used to perform retrovirus-associated antigen (RVAAg), reverse transcriptase activity, and cytopathogenic effect of virus on leukemia cells and normal hematopoietic cells. CPE) and virus particles were tested and analyzed. Results The positive rates of RVAAg in leukemia cell samples before and after culture in 32 patients with AML were 50.0% and 87.5%, respectively. Fourteen RVAAg positive samples simultaneously expressed reverse transcriptase activity and positive XC cell syncytium tests. Electron microscopy confirmed that typical retrovirus particles of type C were found in 75% of AML cell samples. The RVAAg, reverse transcriptase activity, syncytia and electron microscopy of the samples before and after cell culture in 20 normal controls were all negative. Conclusion The leukemia cells in AML patients contain the complete retroviral genome. After being properly induced in vitro, it can be released into the cell by budding in intact virus particles.