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目的通过对HBV-M乙肝血清标志物、乙肝病毒前S1抗原(Pre-S1-Ag)、HBV-DNA之间的对比分析,探讨乙肝病毒前S1抗原检测的临床意义。方法对957例患者血清用酶联免疫法(ELISA)检测HBVPre-S1-Ag和HBV血清标志物,用荧光定量PCR(FQ-PCR)检测HBV-DNA含量。结果 957例乙肝患者中HBV-DNA总检出率为46.8%,HBeAg阳性率为35.6%,Pre-S1-Ag阳性率为79.3%;在HBsAg、HBeAg和HBcAb同时阳性者中,HBV-DNA和Pre-S1-Ag的阳性检出率分别为88.4%和92.1%,差异无统计学意义;616例HBeAg阴性患者中,HBV-DNA和Pre-S1-Ag的阳性率为23.5%和72.2%,两者差异有统计学意义;在HBV-DNA448例阳性标本中,Pre-S1-Ag阳性397例,占88.6%。结论 HBeAg、HBV-DNA和Pre-S1-Ag有较高的符合率,Pre-S1-Ag可作为HBV复制的指标。
Objective To investigate the clinical significance of the detection of pre-S1 antigen of hepatitis B virus (HBV) by comparing the serum HBV-M hepatitis B markers, Pre-S1-Ag and HBV-DNA. Methods Serum samples from 957 patients were tested for serum HBV Pre-S1-Ag and HBV markers by enzyme-linked immunosorbent assay (ELISA) and HBV-DNA levels by fluorescence quantitative PCR (FQ-PCR). Results The total positive rate of HBV-DNA in 957 hepatitis B patients was 46.8%, the positive rate of HBeAg was 35.6%, and the positive rate of Pre-S1-Ag was 79.3%. Among the positive HBsAg, HBeAg and HBcAb positive HBV-DNA and Pre-S1-Ag positive rates were 88.4% and 92.1%, respectively, with no significant difference. The positive rates of HBV-DNA and Pre-S1-Ag in 616 HBeAg negative patients were 23.5% and 72.2% The difference was statistically significant. Among the 488 HBV-DNA positive samples, Pre-S1-Ag was positive in 397 cases (88.6%). Conclusion HBeAg, HBV-DNA and Pre-S1-Ag have high coincidence rate, and Pre-S1-Ag can be used as an indicator of HBV replication.