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目的:研究旋转磁场(RMF)联合氟尿嘧啶(5-FU)对小鼠骨髓瘤细胞(SP2/0)超微弱发光的影响,探讨两者联合对肿瘤细胞的杀伤作用。方法:SP2/0细胞分为四组:对照组,单纯5-FU化疗组(单化组),单纯磁疗组(单磁组),磁场联合5-FU组(磁化组)。用RMF、5-FU作用于SP2/0细胞,化学发光仪进行分析。结果:对照组的化学发光强度值最低,单化组、单磁组、磁化组化学发光强度值均明显高于对照组(P均<0.001)。单磁组低于单化组及磁化组,有明显差别(P<0.001);磁化组的化学发光强度值最高,高于单化组(P<0.001)。结论:磁场和5-FU均可引起SP2/0细胞化学发光强度值的变化,以磁场联合5-FU的化学发光强度值变化最明显,提示磁场单独及联合5-FU对SP2/0细胞有明显的杀伤作用。其机理可能与旋转磁场和5-FU引起细胞自由基增高有关。
Objective: To investigate the effects of rotating magnetic field (RMF) combined with 5-fluorouracil (5-FU) on the ultramicro-luminescence of mouse myeloma cells (SP2 / 0) and to explore the cytotoxicity of these two drugs on tumor cells. Methods: SP2 / 0 cells were divided into four groups: control group, simple 5-FU chemotherapy group (single group), magnetic therapy group (single magnetic group) and magnetic field combined with 5-FU group (magnetization group). Using RMF, 5-FU on SP2 / 0 cells, chemiluminescence analyzer for analysis. Results: The chemiluminescence intensity of the control group was the lowest. The chemiluminescence intensities of the single group, the single magnet group and the magnetization group were significantly higher than those of the control group (all P <0.001). The values of chemiluminescence intensity of the magnetization group were the highest, higher than that of the singulation group (P <0.001). Conclusion: Both magnetic field and 5-FU can cause the change of chemiluminescence intensity of SP2 / 0 cells. The change of chemiluminescence intensity of magnetic field combined with 5-FU shows the most obvious change. It is suggested that the effect of magnetic field and 5-FU on SP2 / 0 cells Clearly killing effect. The mechanism may be related to the rotating magnetic field and 5-FU caused by increased cellular free radicals.