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目的 观察银杏内酯B对大鼠中性白细胞花生四烯酸代谢酶及细胞内钙水平的影响。方法 反相高效液相色谱及Fura 2 /AM荧光指示剂法。结果 银杏内酯B在 0 1- 10 μmol·L-1范围内 ,使花生四烯酸释放量降低10 9% - 2 2 2 % ;0 1- 5 0 μmol·L-1时 ,LTB4 和 5 HETE生成量分别降低 2 9 4% - 88 6 %和 2 6 2 % - 89 3 % ;在0 1- 10 0 μmol·L-1使PAF和fMLP刺激引起的细胞内游离钙浓度分别降低 13 9% - 5 1 4%和 2 2 % - 36 6 %。结论 银杏内酯B能抑制体外大鼠中性白细胞花生四烯酸代谢酶的活性及细胞内游离钙浓度的升高
Objective To observe the effect of ginkgolide B on arachidonic acid metabolic enzymes and intracellular calcium levels in rat neutrophils. Methods Reversed-phase high performance liquid chromatography and Fura 2 /AM fluorescence indicator method. Results Ginkgolide B was in the range of 0 1- 10 μmol·L-1, and the release of arachidonic acid was reduced by 10 9% - 2 2 2 %; 0 5 1 0 0 μmol·L-1, LTB4 and 5 HETE production decreased by 29 4% - 88 6% and 268% - 89 3%, respectively; and the concentration of intracellular free calcium induced by stimulation of PAF and fMLP decreased by 13 - 10 0 μmol·L -1 . % - 5 1 4% and 2 2 % - 36 6 %. Conclusion Ginkgolide B can inhibit the activity of arachidonic acid metabolic enzymes and increase intracellular free calcium concentration in rat neutrophils in vitro.