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目的建立雷公藤多苷片的HPLC指纹图谱,能够全面而有效地控制雷公藤多苷片的质量。方法采用HPLC法,色谱柱为Diamonsil C_(18)分析柱,检测波长218 nm,柱温35℃,以水-乙腈为流动相,流速1.0 mL·min~(-1),梯度冼脱,测定不同生产厂家的8个批号的雷公藤多苷片。结果共出现色谱峰近50个,其中22个为所测样品共有。以单峰面积占总峰面积的百分比≥1%为选择标准,确定了18个峰为共有色谱指纹峰,其总面积占总峰面积的90%以上。结论初步建立了雷公藤多苷片的HPLC指纹图谱,为衡量其质量优劣提供了依据。利用HPLC指纹图谱可以较好地反映雷公藤多苷片的内在质量,且该方法简便、快速、有效、灵敏,有助于雷公藤多苷片的质量控制。
OBJECTIVE To establish the HPLC fingerprinting of Tripterygium wilfordii glycosides tablets, which can control the quality of Tripterygium wilfordii glycosides tablets comprehensively and effectively. Methods The analytical column was Diamonsil C_ (18). The detection wavelength was 218 nm and the column temperature was 35 ℃. The mobile phase consisted of water and acetonitrile with a flow rate of 1.0 mL · min ~ (-1) Different manufacturers of eight batches of Tripterygium glycosides tablets. A total of nearly 50 chromatographic peaks were found, of which 22 were common to the tested samples. Taking the percentage of single peak area to total peak area≥1% as the selection criterion, 18 peaks were identified as the peak of common chromatographic fingerprint, with the total area accounting for more than 90% of the total peak area. Conclusion The HPLC fingerprint of Tripterygium wilfordii glycosides tablets was preliminarily established, which provided the basis for measuring its quality. HPLC fingerprinting can reflect the intrinsic quality of Tripterygium wilfordii glycosides tablets better and the method is simple, rapid, effective and sensitive, which is helpful for the quality control of Tripterygium wilfordii glycosides.