论文部分内容阅读
目的 探讨幽门螺杆菌vacA基因型及其表达产物———VacA与胃十二指肠疾病之间的关系。方法 用聚合酶链反应技术测定 62株从慢性胃炎、消化性溃疡和胃癌患者中分离获得的幽门螺杆菌 (Hp)菌株的vacA基因型 ;用Hela细胞测定Hp菌株体外VacA活性。 结果 62株Hp菌株均含有va cA基因 ,且VacA基因型均为sla/m2型。Hp产生VacA的阳性率为 3 7.1% ,慢性胃炎、消化性溃疡和胃癌患者感染Hp产生VacA的阳性率分别为 3 3 .3 3 %、2 9.17%和 63 .64 %。但是 ,此三种不同疾病患者感染Hp产生VacA的阳性率差异无显著性 (P >0 .0 5 )。 结论 Hp的vacA基因型、VacA并不能作为预示Hp感染个体更有可能发生慢性胃炎或消化性溃疡还是胃癌的可靠指标。本研究未能证实vacA基因型与Hp体外表达VacA的关系。
Objective To investigate the relationship between vacA genotype of Helicobacter pylori and its expression product VacA and gastroduodenal diseases. Methods The vacA genotype of 62 strains of Helicobacter pylori isolated from patients with chronic gastritis, peptic ulcer and gastric cancer was determined by polymerase chain reaction (PCR). The activity of VacA in Hp strains was determined by Hela cells. Results All 62 Hp strains contained va cA gene, and VacA genotype was both sla / m2. The positive rate of VacA produced by Hp was 31.1%. The positive rates of VacA in chronic gastritis, peptic ulcer and gastric cancer were 23.33%, 21.97% and 63.64%, respectively. However, the positive rate of VacA in Hp infected with these three different diseases was not significantly different (P> 0.05). Conclusions The vacA genotype of Hp, VacA, can not be used as a reliable indicator of chronic gastritis or peptic ulcer or gastric cancer in Hp infected individuals. This study failed to confirm the relationship between vacA genotype and VacA in vitro.