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1 材料和方法1.1 材料 人肝癌细胞 SMMC- 772 1由本室保存 ;鼠抗人HSP70单抗、FITC标记的羊抗鼠 Ig G购自 BOSTER公司 ;ENVISION HRP标记的羊抗鼠 Ig G(即用型 )为 DAKO公司产品 .1.2 方法 1细胞的热休克处理 :接种 2× 10 4SMMC- 772 1于置有盖玻片的 2 4孔培养板中 ,37
1 Materials and methods 1.1 Materials Human hepatocarcinoma cells SMMC-772 1 were kept in this chamber; mouse anti-human HSP70 monoclonal antibody, FITC-labeled goat anti-mouse Ig G were purchased from BOSTER; ENVISION HRP-labeled goat anti-mouse Ig G (ready-to-use) ) For DAKO products. 1.2 Heat shock treatment of method 1 cells: inoculate 2 × 10 4 SMMC-772 1 in a 24-well culture plate with coverslips, 37