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栽培种花生是异源四倍体,基因组大,构建花生的分子遗传连锁图谱并对相关性状进行QTL定位研究的工作缓慢。本研究以遗传差异大的亲本组配杂交组合富川大花生×ICG6375构建F2作图群体,采用公开发表的2653对SSR引物,构建了一张含有234个SSR标记、分布于20个连锁群的栽培种花生遗传图谱。该图谱覆盖基因组的长度为1683.43c M,各个连锁群长度在36.11~131.48 c M之间,每个连锁群的标记数在6~15个之间,标记间的平均距离为7.19 c M。结合F3在湖北武汉和阳逻环境下的主茎高和总分枝数鉴定结果,应用Win QTLCart 2.5软件采用复合区间作图法进行了QTL定位和遗传效应分析。共检测到17个与主茎高和总分枝数相关的QTL位点,贡献率在0.10%~10.22%之间,分布于8个连锁群上。综合分析武汉和阳逻环境的鉴定结果,获得重复一致的与主茎高相关的6个QTL,其中q MHA061.1和q MHA062.1位于连锁群LG06上TC1A2~AHGS0153标记区间,贡献率为5.49%~8.95%;q MHA061.2和q MHA062.2位于LG06上AHGS1375~PM377标记区间,贡献率为2.93%~5.83%;q MHA092.2和q MHA091.1位于连锁群LG09上GM2839~EM87标记区间,贡献率为0.53%~9.43%。
Cultivation of peanut is a tetraploid allotetraploid, genome, building peanut molecular genetic linkage map and related traits QTL mapping slow work. In this study, F2 mapping population was constructed from Fucai Peanut × ICG6375 with a large genetic variation in parental combination. Using 2653 pairs of SSR primers published in the study, a cDNA library containing 234 SSR markers distributed in 20 linkage groups Cultivation of peanut genetic map. The length of the genome covered by this map was 1683.43cM, the length of each linkage group was between 36.11 and 131.48 cM, the number of markers per linkage group was between 6 and 15, and the average distance between markers was 7.19 cM. Combining F3 identification results of main stem height and total number of branches in Wuhan and Yangluo of Hubei Province, QTL mapping and genetic effect analysis were carried out by using the method of composite interval mapping with Win QTLCart 2.5 software. A total of 17 QTL loci related to the height of the main stem and the total number of branches were detected, with the contribution rates ranging from 0.10% to 10.22%, distributed on 8 linkage groups. A total of 6 QTLs were found to be consistent with those of the main stems, including q MHA061.1 and q MHA062.1, located in the TC1A2 ~ AHGS0153 marker interval on linkage group LG06 with a contribution rate of 5.49 % ~ 8.95%; q MHA061.2 and q MHA062.2 were located on the marker interval AHGS1375 ~ PM377 on LG06, with a contribution rate of 2.93% -5.83%; q MHA092.2 and q MHA091.1 were located on linkage group LG09 of GM2839 ~ EM87 Interval, the contribution rate of 0.53% ~ 9.43%.