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克隆鉴定甘蔗内源低拷贝基因鲜有研究,影响转基因甘蔗精确鉴定。根据其他作物已有的内标基因和内源低拷贝基因,按照内标基因的物种特异性和通用性原则,用12个有代表性甘蔗栽培品种对11个候选基因进行筛选与鉴定,获得了甘蔗特有的内源低拷贝基因,并以该基因建立了甘蔗内标基因PCR检测技术体系,为转基因甘蔗的精确检测和转基因标准的制定奠定了基础。
Clone identification of endogenous low copy gene sugarcane fresh research, affecting the precise identification of transgenic sugarcane. According to the existing internal standard genes and endogenous low copy genes of other crops, 12 candidate sugarcane cultivars were screened and identified according to the species specificity and generality of internal standard genes. Sugarcane-specific endogenous low-copy gene, and the use of the gene to establish a sugar cane internal standard PCR detection technology system for the precise detection of transgenic sugarcane and transgenic laid the foundation for the development of transgenic standards.