论文部分内容阅读
目的探索一种快速制备常染色体短串联重复序列(short tandem repeat,STR)基因座等位基因分型标准物的简便方法。方法采用PCR-聚丙烯酰胺凝胶电泳(polyacrylamide gel electrophoresis,PAGE)切胶回收-二次PCR-测序的方法制备21号及18号染色STR基因座等位基因分型标准物。结果应用此法制备出大量的基因座等位基因标准物。结论利用PCR-PAGE切胶回收法制备STR基因座等位基因分型标准物是一种简便易行的方法。
Objective To explore a convenient method for rapid preparation of allelic genotypes of short tandem repeat (STR) loci. Methods The allelic ladder alleles of STR loci Nos. 21 and 18 were prepared by polyacrylamide gel electrophoresis (PAGE) gel electrophoresis and secondary PCR-sequencing. Results This method was used to prepare a large number of locus alleles. Conclusion It is a simple and easy method to prepare STR loci allelic typing standards by PCR-PAGE gel cut recovery method.