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目的:研究注射用尖吻蝮蛇血凝酶(Haemocoagulase Agkistrodon,HCA,商品名苏灵)的止血效果及其作用机制。方法:通过体内外形成血栓实验,了解药物的凝血效果及其是否激活凝血因子ⅩⅢ;通过HCA对纤维蛋白原的裂解作用探讨其凝血机制。结果:HCA作用下体内外形成血栓的大小与立止血相差不大,差异无统计学意义;蛋白凝胶电泳图显示HCA作用12 h后纤维蛋白原α亚基带消失;高效液相色谱图显示在8.8 min处有明显的峰出现。结论:HCA具有良好的止血效果,且不激活凝血因子ⅩⅢ;HCA能水解纤维蛋白原的α亚基,裂解出A肽片段(FpA),形成纤维蛋白单体(αBβγ),并聚合成纤维蛋白多聚体,发挥止血作用。
Objective: To study the hemostatic effect of Haemocoagulase Agkistrodon (HCA) and its mechanism of action. Methods: Thrombosis experiment was conducted in vivo and in vitro to understand the coagulation effect of the drug and whether it activated coagulation factor ⅩⅢ. The mechanism of coagulation was explored by cleavage of fibrinogen by HCA. Results: The size of thrombus formed by HCA in vitro and in vivo was similar to that of clonogenic blood, and the difference was not statistically significant. Protein gel electrophoresis showed disappearance of fibrinogen α subunit after HCA treatment for 12 h. High performance liquid chromatography 8.8 min obvious peaks appear. CONCLUSION: HCA has a good hemostatic effect and does not activate coagulation factor XIII. HCA can hydrolyze the α subunit of fibrinogen and cleave A peptide fragment (FpA) to form fibrin monomer (αBβγ) and polymerize into fibrin Multimer, play a role in hemostasis.