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植物在生长发育过程中,包含能量转变、物质转变和形态转变三个方面,而形态转变是通过酶系统来完成的。酶系统包括多种酶类。许多植物含有较多的抗坏血酸,在呼吸代谢过程中,它被氧化成脱氢抗坏血酸,当植物受到损伤时,脱氢酶则失活,脱氢抗坏血酸不再被还原而停留在氧化形式。我们在研究泡桐丛枝病问题的调查中发现,病株的根系大多受过损伤,有的受过机械创伤,有的遭受了虫害,有的受过淹沤等。因此,我们引用了“TTC”法对泡桐的健康植株(简称健株)和丛枝植株脱氢酶的活性,进行了对比测定。现将其方法及结果简介于下: 一、基本原理有关应用“TTC”染色法对种子、根系、花粉活力的测定都有过报道。“TTC”即2,3,5-氯
Plants in the process of growth and development, including energy conversion, material transformation and morphological changes in three areas, and morphological changes through the enzyme system to complete. Enzyme system includes a variety of enzymes. Many plants contain more ascorbic acid, which is oxidized to dehydroascorbate during respiratory metabolism. When plants are damaged, dehydrogenases are inactivated and dehydroascorbate is no longer reduced and remains in oxidized form. In our investigation of the problem of paulownia witches’ broom disease, we found that most of the diseased roots were damaged, some were mechanically injured, others suffered pests and some were flooded. Therefore, we quoted the “TTC” method for paulownia healthy plants (referred to as healthy plants) and the deciduous plants dehydrogenase activity were compared. Now the method and results are briefly described below: First, the basic principles of the application of “TTC” staining of seeds, roots, pollen activity have been reported. “TTC” is 2,3,5-chloro