论文部分内容阅读
阿司匹林,又称乙酰水杨酸,已证实有抑制肿瘤细胞增殖、诱导肿瘤细胞凋亡和抑制血管生成等多种抗癌功能.除已知对环氧合酶COX-2的活性有抑制外,阿司匹林抗癌分子机制尚不十分清楚.已报道阿司匹林可以降低多种癌症发生风险,但应用于人类睾丸肿瘤治疗的研究报道很少.本文研究了阿司匹林对人恶性睾丸肿瘤NTera-2细胞凋亡的机制.通过MTT方法检测细胞活力,发现阿司匹林以时间和剂量依赖方式抑制NTera-2细胞增殖.不同浓度阿司匹林处理NTera-2细胞后,采用Hoechest 33258染色方法和Annexin V-FITC/PI流式法分别检测NTera-2细胞的形态学变化、凋亡小体形成、细胞凋亡水平;RT-PCR结果显示,NTera-2细胞中Fas和caspase-8的表达以阿司匹林剂量依赖性上升;蛋白印迹结果显示,FasL的蛋白表达水平下降并活化caspase-8、caspase-3蛋白表达,PARP出现剪切体.进一步的实验证明,caspase广谱抑制剂Z-VAD-FMK能够减弱阿司匹林诱导NTera-2细胞凋亡.结果显示,阿司匹林能明显抑制NTera-2细胞活力,并通过激活caspase通路诱导NTera-2细胞的凋亡,为进一步利用阿司匹林治疗人类睾丸肿瘤的研究奠定基础.
Aspirin, also known as acetylsalicylic acid, has been shown to inhibit tumor cell proliferation, induce tumor cell apoptosis and inhibit angiogenesis and other anticancer functions.In addition to inhibition of cyclooxygenase COX-2 activity, Aspirin anticancer molecular mechanism is not yet clear.Aspirin has been reported to reduce the risk of a variety of cancers, but there are few reports on the application of aspirin in the treatment of human testicular tumors.In this paper, aspirin on human malignant testicular tumor NTera-2cell apoptosis Mechanism.Purification of NTera-2 cells by aspirin in a time-and dose-dependent manner was observed by MTT assay.After treating NTera-2 cells with different concentrations of aspirin, Hoechst 33258 staining and Annexin V-FITC / PI flow cytometry The morphological changes of NTera-2 cells, the formation of apoptotic bodies and the level of apoptosis were detected by RT-PCR. The expression of Fas and caspase-8 in NTera-2 cells increased in a dose-dependent manner with aspirin. , The protein expression of FasL decreased and the expression of caspase-8 and caspase-3 protein were activated and the cleaved PARP appeared.Further experiments showed that caspase inhibited broad-spectrum Z-VAD-FMK attenuated the apoptosis of NTera-2 cells induced by aspirin.The results showed that aspirin could significantly inhibit the viability of NTera-2 cells and induce the apoptosis of NTera-2 cells by activating the caspase pathway.To further use of aspirin to treat human testis Cancer research laid the foundation.