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目的:观察芪附汤对阿霉素心脏毒性损伤模型大鼠心脏功能的影响,并初步探讨其作用机制。方法:采用心电图仪测定心脏功能的相关参数,采用化学比色法检测心肌细胞超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性及脂质过氧化产物丙二醛(malondialdehyde,MDA)含量。结果:与正常组相比较,模型组大鼠心率显著减慢,QRS波群电压明显降低,Q-T间期明显延长,均有统计学意义(P<0.05),个别出现心律失常;与模型组相比较,芪附汤组、中药对照组心率增加,QRS波群电压和明显增高,Q-T间期显著缩短,均有统计学意义(P<0.05)。与正常组相比较,模型组、芪附汤组、中药对照组心肌细胞SOD和GSH-Px活性减弱,MDA含量上升,均有统计学意义(P<0.05);与模型组相比较,芪附汤组、中药对照组心肌细胞SOD和GSH-Px活性增强,MDA含量下降,均有统计学意义(P<0.05)。结论:芪附汤对阿霉素诱导的大鼠心脏毒性损伤具有保护作用,作用机制与其抗氧化应激有关。
Objective: To observe the effect of Qifu Decoction on cardiac function in adriamycin-induced cardiotoxicity rat model, and to explore its mechanism. Methods: The parameters of cardiac function were measured by electrocardiograph. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in myocardial cells were detected by chemical colorimetry. Lipid peroxidation product malondialdehyde (MDA) content. Results: Compared with the normal group, the heart rate of the model group was significantly slowed down, the QRS wave group voltage was significantly reduced, the QT interval was significantly longer, both statistically significant (P <0.05), individual arrhythmia; and model group phase Compared with the control group, Qifu Decoction group and the traditional Chinese medicine control group showed an increase in heart rate, a significant increase in QRS complex voltage and QT interval, both of which were statistically significant (P <0.05). Compared with the normal group, the activity of SOD and GSH-Px in model group, Qifu Tang group and traditional Chinese medicine control group were decreased and the content of MDA increased (P <0.05). Compared with model group, The activity of SOD and GSH-Px in myocardial tissue of Soup group and traditional Chinese medicine control group increased and MDA content decreased (P <0.05). Conclusion: Qifu decoction has protective effect on doxorubicin-induced cardiotoxicity in rats, and its mechanism is related to its anti-oxidative stress.