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目的探讨组蛋白去乙酰化酶抑制剂SAHA对宫颈癌SiHa细胞的毒性和放射增敏作用。方法 2009年10月至2010年6月在河北联合大学医学院采用MTT法检测SAHA对SiHa细胞的毒性并计算IC50。选择20%IC50的SAHA与放疗联合,克隆形成实验分析SAHA对SiHa细胞的放射增敏作用,采用Sigma Plot 2000 Demo版软件,利用多靶单击模型S=1-(1-eD0/D)n拟合细胞存活曲线,计算放射相关参数和放射增敏比,评价增敏效果。结果 MTT结果显示SAHA对SiHa细胞的毒性反应呈浓度和时间依赖性,SAHA作用SiHa细胞48h的IC50为4.80μmol/L。克隆形成实验结果显示,SAHA联合放疗组细胞的克隆形成率明显低于单独放疗组,二者的平均致死剂量(D0)分别为2.329、1.213,准阈剂量Dq分别为1.721、0.823。放射增敏比(SER)为1.92。结论 SAHA对宫颈癌SiHa细胞有良好的细胞毒性和放射增敏作用。
Objective To investigate the toxicity and radiosensitization effect of SAHA, a histone deacetylase inhibitor, on cervical cancer SiHa cells. Methods From October 2009 to June 2010, the toxicity of SAHA to SiHa cells was detected by MTT assay and the IC50 was calculated in medical school of Hebei Union University. The combination of SAHA with 20% IC50 and radiotherapy was used to analyze the radiosensitization effect of SAHA on SiHa cells by using the Sigma Plot 2000 Demo software. The multi-target click model S = 1- (1-eD0 / D) n The cell survival curve was fitted, and the radiation related parameters and radiosensitization ratio were calculated to evaluate the sensitization effect. Results MTT results showed that the toxicity of SAHA to SiHa cells was concentration-dependent and time-dependent. The IC50 of SAHA-treated SiHa cells for 48 hours was 4.80μmol / L. The results of colony formation assay showed that the colony formation rate of SAHA combined with radiotherapy group was significantly lower than that of radiotherapy group alone. The average lethal dose (D0) of the two groups were 2.329 and 1.223, respectively, and the quasi-threshold dose Dq was 1.721 and 0.823 respectively. The radiosensitization ratio (SER) was 1.92. Conclusion SAHA has good cytotoxicity and radiosensitization to cervical cancer SiHa cells.