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目的探讨静态负荷致骨骼肌损伤与自由基的关系。方法雄性家兔16只,分为2组,分别施加轻、重2种不同姿势的静态负荷,以实验前为自身对照,观察0、2、4、6、8 h血清肌酸激酶(CK)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量的变化。结果重负荷组血清CK、LDH活力高于轻负荷组,差异有统计学意义(P<0.01),随施加负荷时间延长,酶活力逐渐升高,较0 h组比较,差异有统计学意义(P<0.01)。2个负荷组SOD活力的差异无统计学意义(P>0.05);组内比较,随施加负荷时间延长,SOD活力较0h组明显降低,差异有统计学意义(P<0.01)。2个负荷组MDA含量的差异无统计学意义(P>0.05);组内比较,随施加负荷时间延长,MDA含量较0 h组明显增高.差异有统计学意义(P<0.01)。结论长时间静态体位,骨骼肌损伤程度与负荷持续时间、负荷强度有关,损伤原因与脂质过氧化增强和SOD活力降低有关。
Objective To investigate the relationship between static load-induced skeletal muscle injury and free radicals. Methods Sixteen male rabbits were divided into two groups. The static loads of two different postures of light and heavy were applied respectively. Before the experiment, self-control was used to observe the serum creatine kinase (CK) at 0, 2, 4, , Lactate dehydrogenase (LDH), superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. Results The activities of serum CK and LDH in the heavy load group were higher than those in the light load group (P <0.01). The enzyme activity increased gradually with the increase of the load time. Compared with the 0 h group, the difference was statistically significant P <0.01). There was no significant difference in SOD activity between the two load groups (P> 0.05). Compared with the 0h group, the SOD activity in the two load groups was significantly decreased (P <0.01). There was no significant difference in MDA content between the two load groups (P> 0.05). Compared with the 0 h group, the MDA content in the two load groups was significantly increased (P <0.01). Conclusions Long-term static position and degree of skeletal muscle injury are related to duration of load and load intensity. The causes of injury are related to the increase of lipid peroxidation and the decrease of SOD activity.