使用可生物降解聚合物进行蛋白质给药(尤其是重组蛋白质)时,通常采用与普通药物相同的方法进行微囊化。这样在制备过程中,蛋白质药物分子常会接触有机溶媒并受超声等物理因素影响,可能引起蛋白质分子三维结构及构象改变,丧失生物活性。本文选择核糖核酸酶A(RNaseA)为模型蛋白质 When biodegradable polymers are used for protein delivery, in particular recombinant proteins, microencapsulation is usually performed in the same way as conventional drugs. In the process of preparation, protein drug molecules often come into contact with organic solvents and are influenced by ultrasound and other physical factors, which may cause the three-dimensional structure and conformational changes of protein molecules to lose their biological activity. In this paper, RNase A was selected as a model protein