高浓度CO2培养条件下极大螺旋藻光抑制研究

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以极大螺旋藻作为实验材料 ,研究了不同 CO2 浓度培养对螺旋藻光抑制和恢复的影响 ,结果表明由光抑制导致的光合速率下降 ,高浓度 CO2 比低浓度 CO2 培养程度小 ,在高浓度 CO2 条件下培养的极大螺旋藻 ,虽然在强光下也表现出光抑制 ,但与低浓度 CO2 相比 ,光合速率下降得较慢。这种现象在强光与弱光培养均存在 ,但强光培养时更明显。光抑制后的恢复实验表明 ,不同 CO2 浓度培养的极大螺旋藻 ,光系统 光化学活性 (Fv/Fm)在弱光下恢复较好 ,高光强、高浓度 CO2 培养的藻 ,恢复速度稍快 ;而在黑暗中 ,几乎没有恢复 ;在弱光和含氯霉素的条件下 Fv/Fm均下降。由此可见 ,高 CO2 浓度可减轻极大螺旋藻的光抑制 ,但对其光抑制后的恢复影响不大。 The effects of different CO2 concentrations on the inhibition and recovery of spirulina were studied using maximal Spirulina platensis as experimental materials. The results showed that the photosynthesis rate induced by photosystem inhibition decreased, while that of high concentration CO2 was lower than that of low concentration CO2, Spirulina maxima cultivated under CO2 showed photosynthesis inhibition under high light, but its photosynthetic rate decreased more slowly than low CO2 concentration. This phenomenon in both light and weak light culture are present, but more obvious when light training. Recovery experiment after photoinhibition showed that Fv / Fm of the Spirulina maxima cultivated at different CO2 concentrations recovered well in low light, and the recovery speed of the spirulina cultured in high light intensity and high concentration of CO2 was slightly faster. In darkness, there was almost no recovery; Fv / Fm decreased in both low light and chloramphenicol. Thus, high CO2 concentration can reduce the photo-inhibition of Spirulina maxima, but it has little effect on the recovery after photo-inhibition.
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