论文部分内容阅读
分析基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-14(MMP-14)及血管内皮生长因子-C(VEGF-C)在结肠癌中的表达及其与肿瘤临床特征的关系,探讨三者在结肠癌发生发展中的作用及临床意义。采用免疫组织化学方法检测30例结肠癌患者的癌组织及对应癌旁正常结肠组织中MMP-2、MMP-14及VEGF-C的表达情况,分析三者的表达水平与患者性别、年龄、组织学分级、Dukes分期、肿瘤大小、淋巴结转移等临床病理参数之间的相关性。结果显示:1 30例结肠癌组织中MMP-2、MMP-14及VEGF-C的阳性表达率分别为73.3%、80%和66.7%,而在癌旁正常结肠组织中的阳性表达率分别为10%、13.3%和16.7%,两组比较差异均有统计学意义(P<0.05)。2 MMP-2、MMP-14及VEGF-C的表达水平与肿瘤大小、Dukes分期和淋巴结转移有关(P<0.05),而与患者性别、年龄及组织学分级无明显相关性(P>0.05)。3MMP-2、MMP-14及VEGF-C三者两两间表达均相关(P<0.05)。4 30例结肠患者的癌组织中有16例共同高表达MMP-2、MMP-14及VEGF-C,与Dukes分期、病理分级和淋巴结转移有关(P<0.05)。
To analyze the expression of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-14 (MMP-14) and vascular endothelial growth factor-C (VEGF-C) in colon cancer and its relationship with clinical characteristics of tumors. The role and clinical significance of the three in the occurrence and development of colon cancer. Immunohistochemistry was used to detect the expression of MMP-2, MMP-14 and VEGF-C in 30 cases of colorectal cancer and corresponding normal tissue adjacent to the colon. The expression level of the three tissues was analyzed with the patient’s gender, age, and tissue. The correlation between clinical pathological parameters such as grade, Dukes stage, tumor size, and lymph node metastasis was studied. The results showed that the positive rates of MMP-2, MMP-14, and VEGF-C in the 30 cases of colon cancer tissues were 73.3%, 80%, and 66.7%, respectively, while the positive expression rates in the adjacent normal colon tissues were 10%, 13.3% and 16.7%, the difference between the two groups were statistically significant (P <0.05). 2 The expression levels of MMP-2, MMP-14 and VEGF-C were related to tumor size, Dukes stage and lymph node metastasis (P<0.05), but not correlated with gender, age and histological grade (P>0.05). . 3 The expressions of MMP-2, MMP-14 and VEGF-C were all related (P<0.05). Among the 4 30 cases of colon cancer tissues, 16 cases had high expression of MMP-2, MMP-14 and VEGF-C, which was related to Dukes stage, pathological grade and lymph node metastasis (P<0.05).