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目的探讨 B7- 1分子对肿瘤细胞免疫原性的影响。方法将小鼠 B7- 1基因转染的黑色素瘤细胞 ( B16 - m B7.1)与野生型及空载体转染细胞 ( B16 - wt和 B16 - neo)的免疫原性在体内外进行了比较。同源淋巴细胞肿瘤细胞混合培养( MTL Cs)后测定淋巴细胞增殖指数和 CTL s活性 ;将 B16 - neo和 B16 - m B7.1细胞接种于小鼠皮下 ,观察肿瘤生长速度。结果B16 - m B7.1在体外刺激淋巴细胞增殖和诱导 CTL s的能力明显强于对照细胞 ( P<0 .0 5 ) ;尽管 B16 - m B7.1与 B16 - neo细胞在体外增殖能力一致 ,但 B16 - B7.1细胞体内生长速度明显减慢 ( P<0 .0 5 )。结论 B16 - B7.1分子在 B16细胞中表达能增强其免疫原性
Objective To investigate the effect of B7-1 molecule on the immunogenicity of tumor cells. Methods The immunogenicity of mouse B7-1 gene transfected melanoma cells (B16-m B7.1) and wild-type and empty vector transfected cells (B16-wt and B16-neo) were compared in vitro and in vivo. . The lymphocyte proliferation index and CTL s activity were measured after co-culture of homologous lymphocyte tumor cells (MTL Cs); B16-neo and B16-m B7.1 cells were inoculated subcutaneously in mice to observe the tumor growth rate. Results The ability of B16-m B7.1 to stimulate lymphocyte proliferation and induce CTL s in vitro was significantly stronger than that of control cells (P < 0.05), although the proliferation of B16-m B7.1 and B16-neo cells was consistent in vitro. However, the growth rate of B16-B7.1 cells was significantly slower in vivo (P<0.05). Conclusion B16-B7.1 expression in B16 cells enhances its immunogenicity