论文部分内容阅读
目的为了解中国小肠结肠炎耶尔森菌在不同家畜、家禽中的分布规律,对不同地区家畜、家禽进行带菌状况调查。方法采集中国不同地区家畜、家禽咽拭子,肛拭子和粪便标本进行小肠结肠炎耶尔森菌分离培养,并对分离到的菌株进行血清分型、生物分型及毒力相关基因检测。结果猪中小肠结肠炎耶尔森菌携带率最高为12.91%,其次为犬,携带率为9.80%,两者分离的致病性菌株所占比例分别为73.50%和59.44%。而其他动物中小肠结肠炎耶尔森菌携带率较低,鸡、牛和羊分别为4.50%、2.78%和0.89%,且主要以非致病性菌株为主,分别占100%、94.44%和93.33%。结论首次在中国进行大规模家畜、家禽中小肠结肠炎耶尔森菌分布调查。动物中小肠结肠炎耶尔森菌感染率存在较大物种差异和地域性分布特征,猪、犬是小肠结肠炎耶尔森菌的主要储存宿主和传染源,而牛、羊、鸡、鸭等主要携带非致病性小肠结肠炎耶尔森菌,属于偶然宿主。
Objective To understand the distribution of Yersinia enterocolitica in different livestock and poultry in China and investigate the carrying status of livestock and poultry in different regions. Methods Throat swabs, anal swabs and stool samples of domestic animals and poultry from different regions of China were collected for Yersinia enterocolitica isolation and culture. Serotypes, biomarkers and virulence-related genes were detected in the isolates. Results The Yersinia enterocolitica carrying rate in pigs was 12.91%, followed by dogs with carriage rate of 9.80%. The proportion of pathogenic strains isolated from them was 73.50% and 59.44%, respectively. Yersinia enterocolitica carrying rate was 4.50%, 2.78% and 0.89% respectively in other animals, mainly non-pathogenic strains, accounting for 100%, 94.44% And 93.33%. Conclusion The investigation of the distribution of Yersinia enterocolitica in large-scale livestock and poultry in China for the first time was conducted. The prevalence of Yersinia enterocolitica in animals is characterized by large species differences and geographical distribution. Pigs and dogs are the main storage hosts and sources of Yersinia enterocolitica, while cattle, sheep, chickens and ducks Mainly carry non-pathogenic Yersinia enterocolitica, belonging to occasional host.