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目的:考察腺苷钴胺水溶液的光降解动力学特征。方法:运用HPLC法测定水溶液中腺苷钴胺含量及有关物质考察样品浓度、光强度、温度、pH对腺苷钴胺水溶液光稳定性的影响。结果:经线性拟合对比分析,腺苷钴胺溶液的光降解反应级数为n=1,腺苷钴胺溶液对光极敏感,在pH2.5～3.5的溶液中相对稳定,腺苷钴胺溶液的光降解随样品浓度的降低、光强度的升高而增加,恒温避光加速试验中腺苷钴胺溶液的光降解并不随温度的升高而升高,但恒温光照加速试验中温度的升高明显加速腺苷钴胺溶液的光降解。结论:腺苷钴胺溶液光降解属近似一级动力学过程,光降解速率受溶液pH影响显著,热可加速腺苷钴胺溶液的光降解。
OBJECTIVE: To investigate the photodegradation kinetics of adenosine cobalamin aqueous solution. Methods: The content of adenosine and cobalamin in aqueous solution was determined by HPLC. The effects of sample concentration, light intensity, temperature and pH on the photostability of aqueous solution of adenosine cobaltamide were investigated. Results: The results of linear fitting comparison showed that the order of photodegradation of adenosylcobalamin solution was n = 1, the solution of adenosylcobalamin was sensitive to light and was relatively stable in the solution of pH2.5 ~ 3.5. The concentration of adenosine cobalt Photodegradation of amine solution decreased with the decrease of sample concentration and light intensity, and photodegradation of adenosylcobalamin solution did not increase with the increase of temperature in the accelerated light-darkening test. However, in the accelerated light irradiation test, the temperature The increase significantly accelerates the photodegradation of adenosylcobalamin solution. CONCLUSION: Photodegradation of adenosine cobalamine solution is a first-order kinetic process. The photodegradation rate is significantly affected by the pH of the solution, and heat can accelerate the photodegradation of adenosine cobalamin solution.