目的:探讨黄芪甲苷(AS-IV)对高糖受损人脐静脉内皮细胞(HUVECs)分泌基质细胞衍生因子-1α(SDF-1α)、CXC趋化生长因子受体4(CXCR4)的影响，为进一步研究AS-IV通过内皮细胞调节SDF-1α/CXCR4轴改善血管新生奠定基础。方法:从足月健康新生儿脐静脉中分离、培养出HUVECs，采用血管性假性血友病因子(vWF)联合4，6-二脒基-2-苯基吲哚(DAPI)核染鉴定，将获得的HUVECs用含30 mmol/L葡萄糖的EGM-2培养基培养120 h得到高糖受损HUVECs。用不同浓度梯度(25、50、100、200、400 mg/L)AS-IV干预72 h，经酶联免疫吸附法(ELISA)检测SDF-1α和CXCR4含量，以确定AS-IV的最佳作用浓度。用最佳浓度AS-IV干预受损HUVECs，分别于6、12、24、48、72 h收集细胞上清液，经ELISA法检测SDF-1α和CXCR4含量，以确定AS-IV的最佳作用时间。将高糖受损HUVECs随机分为实验组和对照组，同时设置空白组。实验组用最佳浓度AS-IV和最佳时间干预，对照组和空白组用等容量PBS液处理，用ELISA法检测各组SDF-1α和CXCR4含量。结果:胞膜结合vWF因子呈绿色荧光，细胞核DAPI核染后呈蓝色，融合图像显示膜染绿色荧光，核染蓝色荧光，即为HUVECs。100 mg/L的AS-IV作用24 h时，高糖受损HUVECs表达SDF-1α的量达最佳(1 642.87 pg/ml)；50 mg/L的AS-IV作用48 h时，高糖受损HUVECs表达CXCR4的量达最佳(8.44 ng/ml)。与对照组相比，实验组SDF-1α和CXCR4含量明显增多，差异有统计学意义(n P0.05)。n 结论:AS-IV能促进高糖受损HUVECs表达SDF-1α和CXCR4，使其恢复正常生理水平，以发挥修复损伤血管和血管新生作用。“,”Objective:To investigate the effect of astragaloside IV (AS-IV) on the secretion of stromal cell-derived factor-1α (SDF-1α) and CXC chemokine receptor 4 (CXCR4) by high glucose injured human umbilical vein endothelial cells (HUVECs), so as to lay a foundation for further study on AS-IV improving angiogenesis by regulating SDF-1 α/CXCR4 axis of endothelial cells.Methods:HUVECs were isolated and cultured from the umbilical vein of full-term healthy newborns and identified by von Willebrand factor (vWF) combined with 4-diamino-2-phenylindole (DAPI) nuclear staining. The obtained HUVECs was cultured in EGM-2 medium with 30 mmol/L glucose for 120 h to obtain high glucose damaged HUVECs. After intervention with different concentration gradients (25 mg/L, 50 mg/L, 100 mg/L, 200 mg/L, 400 mg/L) AS-IV for 72 hours, the contents of SDF-1α and CXCR4 were detected by enzyme linked immunosorbent assay (ELISA) method to determine the best concentration of AS-IV. The supernatant of damaged HUVECs were collected at 6, 12, 24, 48 and 72 hours after intervention with the best concentration of AS-IV, and the contents of SDF-1α and CXCR4 were detected by ELISA method to determine the best action time of AS-IV. The damaged HUVECs was randomly divided into experimental group and control group, and the blank group was set up at the same time. The experimental group was treated with the best concentration of AS-IV and the best time, the control group and the blank group were treated with the same volume of phosphate buffered saline (PBS) solution, and the contents of SDF-1α and CXCR4 in each group were detected by ELISA method.Results:The vWF factor on the cell membrane was green fluorescence, and the nucleus was blue after DAPI staining. When the fusion image showed green fluorescence, HUVECs were identified by blue fluorescence. The expression of SDF-1α in damaged HUVECs was the best when treated with AS-IV of 100 mg/L for 24 hours (1 642.87 pg/ml), and the expression of CXCR4 in damaged HUVECs was the best when treated with AS-IV of 50 mg/L for 48 hours (8.44 ng/ml). Compared with the control group, the contents of SDF-1α and CXCR4 in the experimental group were significantly increased, and the difference was statistically significant (n P0.05).n Conclusions:AS-IV can promote the expression of SDF-1α and CXCR4 in HUVECs damaged by high glucose to return to normal physiological level, so as to play the role of vascular repair and neovascularization.