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目的探讨胎盘间充质干细胞(p MSCs)缺氧培养液对成纤维细胞及巨噬细胞的作用及机制。方法提取并鉴定胎盘间充质干细胞,ELISA法检测缺氧时p MSCs分泌胰岛素样生长因子1(IGF-1)水平。台盼蓝染色及划痕实验检测p MSCs条件培养液中成纤维细胞增殖及成纤维细胞、巨噬细胞迁移情况。结果成功提取p MSCs,该细胞表达CD29、CD44,不表达CD31、CD34、CD45及HLA-DR。缺氧p MSCs分泌IGF-1增加。p MSCs缺氧培养液中成纤维细胞增殖快于p MSCs常氧培养液(P<0.05)。加入IGF-1Ab后,细胞增殖减慢(P<0.05)。p MSCs缺氧培养液中成纤维细胞或巨噬细胞迁移快于p MSCs常氧培养液(P<0.05)。加入IGF-1Ab后,细胞迁移减慢(P<0.05)。结论 p MSCs缺氧培养液促进成纤维细胞增殖及迁移,促进巨噬细胞迁移。其可能与p MSCs在缺氧培养液中分泌IGF-1增多相关。
Objective To investigate the effect and mechanism of hypoxic medium of placental mesenchymal stem cells (p MSCs) on fibroblasts and macrophages. Methods The placental mesenchymal stem cells were extracted and identified. The levels of insulin-like growth factor 1 (IGF-1) secreted by p MSCs were detected by ELISA. Trypan blue staining and scratch assay were used to detect the proliferation of fibroblasts and the migration of fibroblasts and macrophages in p MSCs conditioned medium. Results p MSCs were successfully extracted. The cells expressed CD29 and CD44 but did not express CD31, CD34, CD45 and HLA-DR. Hypoxia-p MSCs secrete increased IGF-1. The proliferation of fibroblasts in MSCs hypoxic medium was faster than that of normoxic medium (p <0.05). After addition of IGF-1Ab, cell proliferation slowed down (P <0.05). The migration of fibroblasts or macrophages in MSCs hypoxic medium was faster than that in normoxic medium of MSCs (P <0.05). After addition of IGF-1Ab, cell migration slowed down (P <0.05). Conclusion MSCs hypoxic medium can promote the proliferation and migration of fibroblasts and promote the migration of macrophages. It may be related to the increase of IGF-1 secreted by p MSCs in hypoxic culture medium.