论文部分内容阅读
用冷冻法建立家兔角膜内皮损伤的动物模型。将直径为 8mm的铜探头置于液氮中数秒后取出 ,待探头温度显示在 - 12 0℃时将其垂直放于兔角膜中央表面分别接触 5s和 15s。结果显示冷冻区的角膜内皮细胞完全被破坏 ,内皮细胞损伤区与未损伤区之间界限清晰。冷冻 5s和 15s能造成面积相近的角膜内皮缺损 (P >0 .0 5) ,而冷冻 5s者角膜水肿较轻 (P <0 .0 5)。伤后 2 d,内皮缺损面积为(4 .13± 1.97) mm2 ,伤后 3d,一部分的角膜内皮已愈合 ,伤后 4 d所有角膜内皮缺损已被大小不一、形态不规则的再生内皮细胞所覆盖。伤后 12 d,再生的内皮细胞大小趋于均匀一致 ,为多边形。本试验通过对冷冻探头温度的统一监测 ,建立了兔角膜内皮损伤的动物模型 ,并且本模型具有可重复性及简单易行的特点。
Animal models of corneal endothelial injury in rabbits were established by freezing method. A copper probe with a diameter of 8 mm was placed in liquid nitrogen for several seconds and then removed. When the probe temperature was displayed at -12 0 ° C, it was placed vertically on the central corneal surface of the rabbit for 5 s and 15 s, respectively. The results showed that the corneal endothelial cells in the frozen area were completely destroyed, and the boundary between the injured area and the non-injured area of endothelial cells was clear. Frozen 5s and 15s can cause corneal endothelial defects with similar area (P> 0.05), while corneal edema in frozen 5s was lighter (P <0.05). At 2 days after injury, the area of endothelial defect was (4.13 ± 1.97) mm2. After the injury, some corneal endothelium had healed. All the corneal endothelium defects had been damaged 4 days after injury. The irregularly-shaped regenerated endothelial cells Covered. At 12 days after injury, the size of regenerated endothelial cells tended to be uniform and polygonal. In this experiment, the animal model of rabbit corneal endothelial injury was established through the uniform monitoring of the temperature of the freezing probe, and the model was reproducible and easy to operate.