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成团泛菌YS19是从水稻“越富”品种中分离的一种优势内生细菌,与宿主水稻互作时具有多种促生作用,其形成的共质体(symplasmata)结构与菌体抗逆及与宿主互作有重要意义.研究发现了一种在YS19共质体形成阶段高表达的差异蛋白,对其用肽指纹图谱进行鉴定,发现其属于周质空间麦芽糖结合蛋白家族.克隆了该蛋白质的基因,重组表达并分离纯化了该蛋白质,发现它是一种兼职功能蛋白,其不仅参与麦芽糖的ABC运输系统,而且在强酸环境下不易发生变性沉淀,并可通过疏水面的显著暴露结合底物蛋白来发挥分子伴侣活性,这些兼职功能构成了菌体抗逆生存适应性的重要分子基础.
Pantoea agglomerans YS19 is a dominant endophytic bacterium isolated from the rice “Yue-fu” cultivar. It has a variety of promoting effects when interacting with the host rice, and the formed symplasmata structure and bacteria Body resistance and interaction with the host.It was found that a differential protein highly expressed in the YS19 plastid formation stage was identified by peptide fingerprinting and found to belong to the periplasmic space maltose binding protein family. Cloned the gene of the protein, recombinantly expressed and isolated and purified the protein and found that it is a part-time functional protein, which is not only involved in the maltose ABC transport system, but also in a strong acid environment is not prone to denaturation precipitation, and through the hydrophobic surface Significant exposure to binding substrate proteins to exert molecular chaperone activity, these part-time functions constitute an important molecular basis of resilience to bacterial survival.