In the framework of topcolor-assisted technicolor (TC2) theory, we study a flavor changing single top quarkproduction process pp(pp) →Ⅱ0t → tc(tc) at upgraded Tevatron and LHC. The results show that with the flavor changingcoupling of neutral toppion to top and charm quark, toppion provides a large te-channel resonance effect. The signal ofsingle top production could be detected at LHC. Otherwise, the narrow peak in the invariant mass distribution could beclearly detected both at upgraded Tevatron and LHC. Therefore, such single top production process provides a uniqueway to test TC2 model via the detection of tc-channel neutral toppion contribution.