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目的：观察博莱霉素（ＢＬＭ）诱发大鼠肺损伤后肺泡巨噬细胞（ＡＭ）释放肿瘤坏死因子 α（ＴＮＦ α）和白细胞介素 ６（ＩＬ ６）的变化及地塞米松（ＤＭ）对ＡＭ 释放ＴＮＦ α和ＩＬ ６的影响。方法：采用生物活性法测定ＴＮＦ α和ＩＬ ６。结果：灌注ＢＬＭ 后ＡＭ 释放ＴＮＦ α〔（５８．９５±１３．２１）ｋＵ／Ｌ〕和ＩＬ ６〔（３５．００±１０．００）ｋＵ／Ｌ〕明显高于对照组〔ＴＮＦ α为（２３．６３±５．５０）ｋＵ／Ｌ，Ｐ＜ ０．００１；ＩＬ ６ 为（２１．１７±６．０４）ｋＵ／Ｌ，Ｐ＜０．０１〕，ＤＭ 能够抑制ＡＭ 释放ＴＮＦ α和ＩＬ ６，并且与其剂量相关（随ＤＭ 浓度的增加，ＡＭ 分泌ＴＮＦ α和ＩＬ ６ 水平逐渐降低）。ＢＬＭ 组支气管肺泡灌洗液（ＢＡＬＦ）中细胞总数（４．８８±０．６５）×１０６ 也明显高于对照组〔（１．４７±０．４１）×１０６，Ｐ＜ ０．００１〕。结论：ＤＭ 能以剂量依赖方式抑制ＡＭ 释放ＴＮＦ α和ＩＬ ６
Objective: To observe the changes of tumor necrosis factor α (TNF α) and interleukin 6 (IL 6) released from alveolar macrophages (AM) after bleomycin (BLM) -induced lung injury in rats and the effect of dexamethasone (DM) On the release of TNF alpha and IL 6 by AM. Methods: The biological activity of TNF α and IL 6 were measured. Results: TNF α 〔(58.95 ± 13.21) kU / L〕 and IL 6 [(35.00 ± 10.00) kU / L〕 were significantly higher in AM group than those in control group [TNFα was 23.63 ± 5.50) kU / L, P <0.001; IL 6 was (21.17 ± 6.04) kU / L, P <0.01〕 6, and its dose-related (with the DM concentration increased, AM secretion of TNF α and IL 6 levels gradually decreased). The total number of cells (4.88 ± 0.65) × 106 in BLM group was significantly higher than that in control group (1.47 ± 0.41) × 106, P <0.001. Conclusion: DM can inhibit the release of TNF α and IL 6 by AM in a dose - dependent manner