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目的探讨依那普利是否具有抑制同型半胱氨酸(Hcy)诱导的大鼠血管平滑肌细胞(VSMCs)表型转化的作用及其可能的信号通路。方法 SD大鼠主动脉VSMCs原代细胞培养鉴定,取4~7代VSMCs分为对照组、100μmol/L同型半胱氨酸组、同型半胱氨酸+依那普利干预组和Hcy+LY-294002干预组。采用M TT法检测各组VSMCs的增殖情况;采用划痕法和Transwell法检测各组VSMCs的迁移情况;ICC法观察各组VSMCs的细胞形态;采用Western blotting法检测各组VSMCs中SM-actin、SM-MHC、calponin、OPN和p AKT的表达。结果与对照组相比,同型半胱氨酸组VSMCs增殖迁移增加,细胞形态变圆,SM-MCH和calponin表达减少(P<0.01),OPN和p-AKT表达增加(P<0.01)。与Hcy组相比,依那普利组和LY-294002组VSM Cs增殖和迁移减少,细胞形态变得细长,SM-MHC和Calponin表达增加(P<0.01);OPN和p AKT表达减少(P<0.01)。结论 Hcy可以促进VSMCs表型转化,依那普利可以抑制Hcy诱导的VSMCs表型转化,其机制可能是通过抑制PI3K/p-AKT途径实现。
Objective To investigate whether enalapril can inhibit homocysteine (Hcy) -induced phenotypic transformation of rat vascular smooth muscle cells (VSMCs) and its possible signaling pathways. Methods The primary cultured VSMCs of SD rats were identified and cultured. The VSMCs from 4 to 7 passages were divided into control group, 100μmol / L homocysteine group, Hcy + enalapril intervention group and Hcy + LY -294002 intervention group. The proliferation of VSMCs in each group was detected by MTT method. The migration of VSMCs in each group was detected by scratch method and Transwell method. The morphology of VSMCs in each group was observed by ICC method. The expressions of SM-actin, SM-MHC, calponin, OPN and p AKT expression. Results Compared with the control group, the proliferation and migration of VSMCs in the homocysteine groups were increased, the morphology of the cells was round, the expressions of SM-MCH and calponin were decreased, and the expressions of OPN and p-AKT were increased (P <0.01). Compared with Hcy group, the proliferation and migration of VSMCs in enalapril group and LY-294002 group decreased, the morphology of cells became slender, the expression of SM-MHC and Calponin increased (P <0.01), and the expression of OPN and p AKT decreased P <0.01). CONCLUSION: Hcy can promote the phenotypic transformation of VSMCs. Enalapril can inhibit Hcy-induced VSMCs phenotypic transformation by inhibiting the PI3K / p-AKT pathway.