采用CO_2电极测定血清TCO_2,并对其方法学进行初步研究。CO_2电极法测定血清TCO_2批内精密度为:2.03％,1.94％和1.91％;批间精密度:2.44％,2.31％和2.28％;日间精密度为:2.77％,2.79％和2.57％;总精密度为:3.65％,3.39％和3.16％。血清TCO_2。含量在5～50mmol/L时线性良好,回归方程为:Y=1.0032×-0.08749。平均回收率为:100.40％。轻微黄疸,溶血和脂浊对测定无影响,叠氮钠对测定有正干扰。电极法测定血清TCO_2准确、快速,适合临床应用。 The CO_2 electrode was used to measure the serum TCO_2, and its methodology was preliminary studied. The intra-assay precision of CO_2 electrode method was 2.03%, 1.94% and 1.91%, respectively; the inter-batch precision was 2.44%, 2.31% and 2.28%; the intraday precision was 2.77%, 2.79% and 2.57% The total precision is: 3.65%, 3.39% and 3.16%. Serum TCO_2. The content of 5 ~ 50mmol / L was good linear regression equation was: Y = 1.0032 × -0.08749. The average recovery was: 100.40%. Mild jaundice, hemolysis and lipid cloud no effect on the determination of sodium azide has positive interference. Electrode method for the determination of serum TCO 2 is accurate, rapid and suitable for clinical application.