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目的 通过动物实验探讨肺动脉血栓放射免疫显像在肺栓塞诊断及血栓性质鉴别中的价值。方法 选取杂种犬 7只 ,制成肺动脉血栓栓塞模型。静脉注射99Tcm 标记的活化血小板单克隆抗体SZ 5 1,进行活体γ显像。完整取出心肺后行离体心肺显像。分别观察血栓部位及周围组织的放射性分布 ,计算栓塞部位与周围组织的放射性比值。随后取出血栓及周围部分组织 ,称重后测定单位质量的放射性计数及单位质量血栓与周围组织的放射性比值。结果 注入显像剂后 1h ,血栓部位开始显影并逐渐清晰。新鲜血栓放射性摄取明显高于周围肺组织 ,注入显像剂后 3h ,活体显像和完整心肺离体显像新鲜血栓与周围肺组织的放射性比值分别为 2 .8± 0 .6和 3.5± 1.2。形成 1d的新鲜血栓的放射性摄取明显高于形成 1周左右的血栓 ,单位质量新鲜血栓与周围组织的放射性比值达 17.5± 1.3,是形成 1周左右血栓的 2 .6倍。结论 99Tcm SZ 5 1肺动脉血栓放射免疫显像对诊断急性肺栓塞的形成及鉴别新鲜与陈旧血栓具有较好的应用前景。
Objective To investigate the value of radioimmunoimaging pulmonary embolism in the diagnosis of pulmonary embolism and the identification of thrombus by animal experiments. Methods Seven dogs were selected to make pulmonary thromboembolism model. Intravenous 99Tcm labeled activated platelet monoclonal antibody SZ 5 1, for in vivo γ imaging. Complete removal of cardiopulmonary cardiopulmonary imaging. Respectively observe the thrombus site and the surrounding tissue radioactivity distribution, calculate the radioactive value of the embolization site and the surrounding tissue. Then remove the thrombus and the surrounding part of the organization, weighed after the determination of unit mass radioactivity counts and unit mass thrombus and surrounding tissue radioactivity ratio. Results 1h after injecting the imaging agent, thrombus began to develop and gradually clear. Fresh thrombus radioactivity uptake was significantly higher than the surrounding lung tissue, 3h injection of imaging agent, live imaging and complete cardiopulmonary imaging of fresh thrombus and surrounding lung tissue radioactivity ratio were2.8 ± 0.6 and 3.5 ± 1.2 . The formation of fresh thrombosis 1d radioactive intake was significantly higher than the formation of thrombosis about 1 week, the unit mass of fresh thrombus and surrounding tissue radioactivity ratio of 17.5 ± 1.3, is the formation of thrombosis about 1 week 2.6 times. Conclusion 99Tcm SZ 5 1 pulmonary arterial thrombosis radioimmunoimaging has a good application prospect in the diagnosis of acute pulmonary embolism and the identification of fresh and old thrombus.