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利用交替三线性分解算法与反相高效液相色谱 二极管阵列检测 (RP HPLC DAD)相结合 ,在色谱洗脱时间为1 0 86min～ 1 399min(间隔 1 / 1 50min)、紫外吸收波长为 2 68nm～ 2 98nm(间隔 1nm)时对苯二酚位置异构体的重叠色谱及光谱体系进行了分辨研究。分辨结果与实际结果一致。同时测定了水溶液中共存的邻苯二酚、间苯二酚和对苯二酚的含量 ,回收率分别为 (1 0 0 1± 1 0 ) % ,(99 4± 1 4) % ,(1 0 0 5± 1 7) %。研究结果表明 :该方法定量快速准确 ,实验操作步骤简单 ,解决了在干扰物存在条件下三者很难同时分辨的问题 ,说明三线性分解算法能有效地解决HPLC DAD数据中的二阶校正问题
Using the combination of alternate trilinear decomposition and reversed-phase high performance liquid chromatography (HPLC) diode array detection (RP HPLC DAD), the chromatographic elution time was 1.086min-1 399min (interval 1 / 150min) and the UV absorption wavelength was 682nm ~ 2 98nm (1nm interval) when the hydroquinone position isomers by overlapping chromatography and spectroscopic systems were resolved. The resolution result is consistent with the actual result. The contents of catechol, resorcinol and hydroquinone coexisted in aqueous solution were determined simultaneously. The recoveries were (100 ± 1 0)%, (99 4 ± 1 4)%, (1 0 0 5 ± 1 7)%. The results show that the proposed method is fast, accurate and accurate. The experimental procedure is simple, which solves the problem that it is difficult to distinguish the three in the presence of interfering substances. It shows that the trilinear decomposition algorithm can effectively solve the second-order calibration problem in HPLC DAD data