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A new tetradecapeptide, ranamargarin, has been isolated by Sep-Pak C<sub>18</sub> and HPLC from methanol extracts of the skin of the Chinese frogRana margaratae. The sequence of the peptide is: Asp-Asp-Ala-Ser-Asp-Arg-Ala-Lys-Lys-Phe-Tyr-Gly-L