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目的：研究雷公藤内酯醇对Ｔ淋巴细胞核因子－κＢ（ＮＦ－κＢ）活力的影响，进一步研究雷公藤内酯醇免疫抑制作用的机制。方法：利用凝胶迁移率实验（ＥＭＳＡ）检测不同浓度的雷公藤内酯醇对于普通培养状态下和同时使用佛波脂／植物血凝素（ＰＭＡ／ＰＨＡ）激活的Ｊｕｒｋａｔ细胞中ＮＦ－κＢ活力的影响。结果：在普通培养状态下和同时使用ＰＭＡ／ＰＨＡ激活的Ｊｕｒｋａｔ细胞中均存在一定活力的ＮＦ－κＢ，使用ＰＭＡ／ＰＨＡ处理可使Ｊｕｒｋａｔ细胞中ＮＦ－κＢ的活力显著的升高，雷公藤内酯醇可以降低两种培养状况下的Ｊｕｒｋａｔ细胞中ＮＦ－κＢ活力，并以激活状态下更为显著。结论：雷公藤内酯醇降低Ｔ淋巴细胞中ＮＦ－κＢ活力的作用可能是其免疫抑制效应的分子机制之一。
Objective: To study the effects of triptolide on the activity of nuclear factor-κB (NF-κB) in T lymphocytes and to study the mechanism of triptolide’s immunosuppression. METHODS: The effects of different concentrations of triptolide on NF-κB activity in Jurkat cells activated by phorbol myristate / phytohaemagglutinin (PMA / PHA) under ordinary culture conditions were examined by gel shift assay (EMSA) influences. RESULTS: NF-κB was found in Jurkat cells activated by PMA / PHA under ordinary culture conditions. The activity of NF-κB in Jurkat cells was markedly increased by PMA / PHA treatment. The activity of triptolide Alcohols reduced NF-κB activity in Jurkat cells under both culture conditions and were more pronounced in the activated state. Conclusion: The effect of triptolide on NF-κB activity in T lymphocytes may be one of the molecular mechanisms of its immunosuppressive effects.