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【目的】观察脾肾阳虚状态下大鼠脾脏中主要Ⅰ相和Ⅱ相药物代谢酶活性的改变。【方法】采用连续17 d灌胃大黄水煎液同时注射氢化可的松的方法复制大鼠脾肾阳虚模型,观察脾脏中6种Ⅰ相代谢酶(CYP2C19、CYP2D6、CYP2C9、CYP1A2、CYP2C8、CYP3A4)和4种Ⅱ相代谢酶[苯酚磺基转移酶(PST)、尿苷二磷酸葡醛酸转移酶1(UGT1)、谷胱甘肽转移酶(GST)、雌激素硫酸转移酶(SULT1E1)]的活性变化。【结果】模型组PST、UGT1、GST、SULTl E1活性显著低于正常组(P<0.05或P<0.01);CYP1A2活性显著高于正常组(P<0.01),而CYP2C19、CYP2D6、CYP2C8、CYP3A4、CYP2C9等酶的活性无显著变化(P>0.05)。【结论】脾肾阳虚状态下脾脏药物代谢酶特别是II相代谢酶的活性会发生显著改变。
【Aim】 To observe the changes of major metabolic enzymes in the spleen of rats with spleen-kidney-yang deficiency. 【Method】 The model of spleen-kidney-yang deficiency was established by intragastric administration of Rhubarb decoction for 17 days and hydrocortisone injection simultaneously. Six phase Ⅰ metabolizing enzymes (CYP2C19, CYP2D6, CYP2C9, CYP1A2, CYP2C8, CYP3A4) and four phase Ⅱ metabolic enzymes (PST, UGT1, GST, SULT1E1 )] Activity changes. 【Results】 The activities of PST, UGT1, GST and SULT1 E1 in the model group were significantly lower than those in the normal group (P <0.05 or P <0.01). The activity of CYP1A2 in the model group was significantly higher than that in the normal group (P <0.01), while CYP2C19, CYP2D6, CYP2C8, CYP3A4 , CYP2C9 other enzymes activity no significant change (P> 0.05). 【Conclusions】 The activities of spleen drug metabolizing enzymes, especially phase II metabolizing enzymes, may change significantly under the state of spleen and kidney yang deficiency.