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目的:探讨伯基特淋巴瘤(BL)的临床病理特征、免疫表型及鉴别诊断。方法:运用组织病理学、免疫组织化学和EBER原位杂交,对13例BL及7例伯基特样淋巴瘤(BLL)患者的肿瘤组织进行检测,并复习相关文献。结果:20例散发性病例中,病变部位在淋巴结内者,位于颈部淋巴结6例,颌下淋巴结4例,锁骨下淋巴结1例,腋下淋巴结1例;病变部位在淋巴结外者,位于回盲部3例,精索、睾丸、结肠、肌肉、前列腺各1例。光镜下可见瘤组织由单一的、弥漫浸润的中等大小圆细胞组成,其中散在分布着吞噬细胞碎片的组织细胞,呈“星空”样改变。免疫表型检测示,瘤细胞弥漫表达CD20和CD79α,部分表达CD10,>90%的瘤细胞Ki-67阳性,而CD3、CD43、bcl-2和TdT呈阴性。EBER原位杂交发现,3例瘤细胞呈阳性。结论:散发性BL属高度恶性肿瘤,确诊依赖于病理组织学与免疫组化标记。
Objective: To investigate the clinicopathological features, immunophenotype and differential diagnosis of Burkitt’s lymphoma (BL). METHODS: Tumor tissues from 13 patients with BL and 7 with Burkitt-like lymphoma (BLL) were examined by histopathology, immunohistochemistry and EBER in situ hybridization. The relevant literature was reviewed. Results: In 20 cases of sporadic cases, the lesion was located in the lymph nodes, located in the cervical lymph nodes in 6 cases, submandibular lymph nodes in 4 cases, 1 cases of subclavian lymph nodes, axillary lymph nodes in 1 case; lesions in the lymph nodes, located in the back Blind in 3 cases, spermatic cord, testis, colon, muscle, prostate in 1 case. Light microscopy showed that tumor tissue consists of a single, diffuse infiltration of medium-sized round cells, which scattered in the phagocyte debris of tissue cells, was “starry sky” -like changes. Immunophenotyping showed that tumor cells diffusely express CD20 and CD79α, partially express CD10, and> 90% of tumor cells have positive Ki-67, while CD3, CD43, bcl-2 and TdT are negative. EBER in situ hybridization found that 3 cases of tumor cells were positive. Conclusion: Sporadic BL is a highly malignant tumor. The diagnosis depends on histopathology and immunohistochemistry.