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建立了番茄子叶高频再生体系,优化了农杆菌介导的外源基因转化番茄的条件,将IPT和etr1 1双基因导入番茄中,以除草剂PPT作为筛选标记,得到3株转化再生植株.通过PCR,RT PCR检测证明etr1 1和IPT基因已整合到转基因番茄植株的基因组中,并在转录水平有一定表达.
A high-frequency regeneration system of tomato cotyledons was established. Agrobacterium-mediated transformation of exogenous gene into tomato was optimized. Three IPT and etr1 1 genes were introduced into tomato and PPT as herbicide marker. Three transformed plants were obtained. The results of PCR and RT-PCR showed that etr1 1 and IPT genes were integrated into the genome of transgenic tomato plants and expressed at the transcriptional level.