目的　明确本院烧伤病房不同患者中连续分离到的高度多重耐药绿脓假单胞菌是否由同一起源的菌株传播。方法　用浓度梯度法测定绿脓假单胞菌的最小抑菌浓度 ,通过重复序列引物聚合酶链反应 (rep PCR) ,用肠杆菌科基因重复序列和噬菌体重复序两种引物分别对绿脓假单胞菌进行基因分型。结果　抗生素敏感试验显示 2 3株绿脓假单胞菌中 13株对 12种抗菌药物均耐药 ,6株仅对哌拉西林 /他唑巴坦敏感 ,对头孢哌酮 /舒巴坦中介 ,而对其他 10种抗菌药物高度耐药 ,另 4株为敏感菌 (耐药的抗菌药物≤ 4种 )。多重耐药菌株的rep PCR产物经琼脂糖电泳分析 ,其基因型完全相同 ,并与敏感株之间有明显区别。结论　烧伤病房高度多重耐药绿脓假单胞菌的流行是由同一克隆菌株传播所致 Objective To determine whether the highly isolated multi-drug resistant Pseudomonas aeruginosa from different patients in our hospital’s burn ward was transmitted by the same strain of origin. Methods The minimum inhibitory concentration (MIC) of Pseudomonas aeruginosa was determined by concentration gradient method. Two kinds of primers of Enterobacteriaceae and phage repeat were used to detect the minimum inhibitory concentration Aeromonas for genotyping. Results Antibiotic susceptibility test showed that 13 out of 23 strains of Pseudomonas aeruginosa were resistant to 12 antibiotics, 6 were sensitive to piperacillin / tazobactam, While the other 10 kinds of antimicrobial resistance, the other 4 strains of sensitive bacteria (drug-resistant antibiotics ≤ 4 species). The rep PCR products of multidrug-resistant strains were analyzed by agarose gel electrophoresis and their genotypes were identical and significantly different from those of the susceptible strains. Conclusion The prevalence of highly multi-drug resistant Pseudomonas aeruginosa in burn ward was caused by the spread of the same clonal strain