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本文研究不同浓度的氮、磷和碳以及不同的氮源对螺旋藻脂肪酸组成和含量的影响 .结果表明 :改变营养盐的浓度对藻体TSFA和TPUFA均有较大影响 ,而对TMUFA无显著影响 ;氮源NaNO3浓度为 0 .5 g dm3、磷源K2 HPO4 浓度为 0 .3～ 0 .5g dm3、碳源NaHCO3浓度为 4.5 g dm3时 ,藻体均可合成亚麻酸C1 8∶3,藻体TPUFA有最大值分别为 44.0 7%、46 .47%和 5 1 .98% ;以尿素和硫酸铵为氮源 ,藻体含有C1 6∶2、TPUFA分别可提高 4.46 %和 7.2 8% .以谷氨酸为氮源 ,藻体含有C1 6∶2和C1 8∶3、TPUFA可提高 6 .43%
In this paper, the effects of different concentrations of nitrogen, phosphorus and carbon and different nitrogen sources on the composition and content of fatty acids in spirulina were studied.The results showed that: the concentration of nutrients had a significant effect on TSFA and TPUFA, but no significant effect on TMUFA , When the concentration of nitrogen source is 0.5 g dm3, the concentration of K2 HPO4 is 0.3-3.5 g dm3, and the concentration of carbon source is 4.5 g dm3, the algae can synthesize linoleic acid C1 8: 3, The highest value of phytoplankton TPUFA was 44.0 7%, 46.47% and 51.98% respectively. With urea and ammonium sulfate as nitrogen source, the algae contained C1 6: 2 and TPUFA increased by 4.46% and 7.28% Using glutamate as nitrogen source, the algae contained C1 6: 2 and C1 8: 3, TPUFA increased by 6 .43%