制备有效的疟疾疫苗需用纯的疟原虫裂殖体或裂殖子,过去报道用一些梯度分离法取得了成功。本文用碘葡酰胺作等密度离心方法分离连续培养的恶性疟原虫裂殖体也取得了成功。所用的恶性疟原虫系Camp株,以改良的Trager与Jensen法在体外连续培养保种,并以山梨醇作两次处理使原虫同步。密度梯度与分离所用的介质为3.5g碘葡酰胺粉,加入重蒸水溶解至10ml,过滤除菌后置 The preparation of an effective malaria vaccine requires the use of pure Plasmodium schizonts or merozoites, as reported in the past with some gradient separations. In this paper, Iodoglutamyl for iso-density centrifugation method for continuous culture of Plasmodium falciparum schizonts has also been successful. The P. falciparum Camp strain used was maintained in vitro by a modified Trager and Jensen method in vitro for continuous culture and synchronized with sorbitol for two treatments. The medium used for density gradient and separation was 3.5 g iodoglucan powder, dissolved in 10 ml distilled water, filtered and sterilized