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目的建立清肠栓(三七、青黛、五倍子等)的质量标准。方法 TLC法对三七、青黛、五倍子和马齿苋进行定性鉴别,HPLC法定量测定三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1含有量。结果 TLC斑点清晰,分离度好。三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1分别在1.007 5~8.06μg(r=0.999 9)、0.99~7.92μg(r=0.999 1)、1.012 5~8.1μg(r=0.999 1)范围内线性关系良好,平均回收率均大于95%。结论该方法可靠准确,专属性好,可用于清肠栓的质量控制。
Objective To establish the quality standard of Qingchang suppository (Panax notoginseng, indigo, gallnut, etc.). Methods TLC method was used to qualitatively identify Panax notoginseng, indigo, gallnut and purslane, and the content of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 were quantitatively determined by HPLC. Results TLC spots clear, good resolution. The concentrations of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 were in the range of 1.007 5 ~ 8.06μg (r = 0.999 9), 0.99 ~ 7.92μg (r = 0.999 1), 1.012 5 ~ 8.1μg (r = 0.999 1) Good linearity, the average recovery rate was greater than 95%. Conclusion The method is reliable, accurate and specific and can be used for the quality control of Qingchang suppository.