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目的:探讨ABCG2和OCT4在胆囊癌组织中的表达及临床意义。方法:应用实时荧光定量PCR法检测新鲜的胆囊良、恶性组织中ABCG2和OCT4 mRNA中的表达。采用Western印迹法检测新鲜的胆囊良、恶性组织中ABCG2和OCT4蛋白的表达。采用免疫组化法检测40例胆囊癌组织和8例慢性胆囊炎组织石蜡切片中ABCG2和OCT4的表达水平。对ABCG2和OCT4表达情况进行统计学处理,并分析其与临床病理特征之间的关系。结果:①实时荧光定量PCR显示,胆囊癌组织中ABCG2和OCT4 mRNA中的表达分别为(117.9±10.5)和(86.2±13.2),均高于慢性胆囊炎组织[(27.1±7.9)和(2.1±1.0)]的表达,各组间有统计学差异。②Western印迹法检测显示,ABCG2蛋白在胆囊癌组织中的表达水平较强,而在慢性胆囊炎组织表达较弱;在慢性胆囊炎组织中未见OCT4蛋白表达,而在肿瘤中都有表达。③免疫组化实验结果显示,ABCG2的阳性表达率在胆囊癌和慢性胆囊炎组分别为60.0%和37.5%,两组间有统计学差异(P<0.05)。④OCT4在慢性胆囊炎组织中不表达;在胆囊癌组织中,OCT4表达阳性率为45%。⑤胆囊癌组织中,ABCG2和OCT4表达水平在年龄、性别方面无统计学差异(P>0.05),但与周围脏器侵犯与否、癌组织分化程度及病理TNM分期有关(P<0.05)。⑥ABCG2与OCT4在胆囊癌组织中的表达呈正相关(rs=0.65,P<0.05)。结论:与慢性胆囊炎组织相比,ABCG2和OCT4在胆囊癌组织中的转录和翻译水平都有过表达。ABCG2和OCT4的表达水平与胆囊癌侵袭能力、分化程度及TNM分期有关,且两者表达呈正相关;提示ABCG2和OCT4是反映胆囊癌生物学行为的重要指标。
Objective: To investigate the expression and clinical significance of ABCG2 and OCT4 in gallbladder carcinoma. Methods: The expression of ABCG2 and OCT4 mRNA in fresh and benign gallbladder tissues was detected by real-time fluorescence quantitative PCR. Western blotting was used to detect the expression of ABCG2 and OCT4 protein in fresh and benign gallbladder tissues. Immunohistochemistry was used to detect the expression of ABCG2 and OCT4 in 40 cases of gallbladder carcinoma and 8 cases of chronic cholecystitis. The expression of ABCG2 and OCT4 were statistically analyzed, and analyzed the relationship between clinicopathological features. Real-time quantitative PCR showed that the expressions of ABCG2 and OCT4 mRNA in gallbladder carcinoma tissues were (117.9 ± 10.5) and (86.2 ± 13.2), respectively, higher than those in chronic cholecystitis tissues [(27.1 ± 7.9) and (2.1 ± 1.0)], there was a significant difference among the groups. ②Western blotting showed that ABCG2 protein expressed strongly in gallbladder carcinomas and weakest in chronic cholecystitis tissues. No OCT4 protein was found in chronic cholecystitis tissues, but was expressed in all tumors. ③ The results of immunohistochemistry showed that the positive expression rate of ABCG2 in gallbladder cancer and chronic cholecystitis group were 60.0% and 37.5% respectively, with statistical significance (P <0.05). ④ OCT4 is not expressed in chronic cholecystitis tissues; in gallbladder cancer tissues, the positive rate of OCT4 expression is 45%. (5) The expression of ABCG2 and OCT4 in gallbladder cancer tissues was not significantly different in age and sex (P> 0.05), but related to the invasion of surrounding organs, the differentiation of cancer tissues and the TNM stage (P <0.05). ⑥ ABCG2 and OCT4 expression in gallbladder cancer was positively correlated (rs = 0.65, P <0.05). CONCLUSIONS: ABCG2 and OCT4 are overexpressed in gallbladder carcinomas as compared with chronic cholecystitis. The expression levels of ABCG2 and OCT4 were related to the invasive ability, differentiation degree and TNM stage of gallbladder carcinoma, and the expressions of ABCG2 and OCT4 were positively correlated. The results suggested that ABCG2 and OCT4 are important indicators of the biological behavior of gallbladder carcinoma.