论文部分内容阅读
目的研究扩张型心肌病(DCM)患者血清中抗β1肾上腺素能受体(β1-AR)自身抗体的生物学效应及其作用机制。方法采用酶联免疫吸附测定(SA-ELISA)技术,检测39例DCM患者和97例正常人血清标本中的抗β1-AR自身抗体;利用亲和层析法纯化抗体阳性和阴性血清中的IgG,观察抗β1-AR自身抗体对乳鼠心肌细胞搏动频率和成年大鼠心肌细胞腺苷酸环化酶活性的影响。结果DCM患者血清中抗β1-AR自身抗体的阳性率和抗体滴度明显高于正常人;纯化的抗β1-AR自身抗体IgG既可增加乳鼠心肌细胞的搏动频率,又可增强成鼠心肌细胞腺苷酸环化酶的活性,使cAMP产生量增加。结论DCM患者血清中的抗β1-AR自身抗体对心脏的β1-AR具有激动剂样正性变时效应,该自身抗体可能通过β1AR-Gs-AC-cAMP-PKA信号通路起作用。
Objective To study the biological effects of anti-β1-adrenergic receptor (β1-AR) autoantibodies in the serum of patients with dilated cardiomyopathy (DCM) and its mechanism. Methods Anti-β1-AR autoantibodies were detected by enzyme-linked immunosorbent assay (SA-ELISA) in serum samples of 39 patients with DCM and 97 normal controls. The IgG in serum was purified by affinity chromatography To observe the anti-β1-AR autoantibodies on neonatal rat cardiomyocytes pulsatility and adult rat myocardial adenylate cyclase activity. Results The positive rate and antibody titers of anti-β1-AR autoantibodies in DCM patients were significantly higher than those in normal controls. The purified anti-β1-AR autoantibodies IgG could increase the frequency of cardiomyocytes pulsation, Cellular adenylate cyclase activity, increased cAMP production. CONCLUSION: Anti-β1-AR autoantibodies in the serum of patients with DCM have agonist-like positive anaplastic effects on β1-AR in the heart, and this autoantibodies may act through the β1AR-Gs-AC-cAMP-PKA signaling pathway.