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目的渤海海泥来源放线菌Streptomyces sp.LXF-80可以生产罕见的包含α-吡喃酮的三环骈合聚酮化合物肠道菌素(Enterocin)。美替拉酮是1种细胞色素P450氧化酶的抑制剂,本实验试图利用美替拉酮影响肠道菌素的生合成过程,表达获得新的聚酮类化合物。方法向LXF-80的发酵培养基中添加酶抑制剂,结合HPLC分析确定最优条件,并进行大规模发酵和目标产物的分离。结果从添加美替拉酮的LXF-80发酵提取物中共分离获得3个化合物,包括Enterocin(1)、5-deoxyenterocin(2)以及3-epi-5-deoxyenterocin(3)。结论实验结果证明美替拉酮的添加,影响了菌株聚酮类化合物的生合成过程,得到2个肠道菌素的形成过程中的中间体化合物2和3。
Objective Streptomyces sp. LXF-80, a marine source-derived actinomycete in the Bohai Sea, can produce the rare tricyclic polyketide enterocin (Enterocin) containing α-pyrone. Mesotrione is a cytochrome P450 oxidase inhibitor, the experiment attempts to use of metyrapone affect intestinal biotin synthesis process, the expression of new polyketide. Methods Enzyme inhibitors were added into the fermentation medium of LXF-80, and the optimal conditions were determined by HPLC analysis. The fermentation was carried out on large scale and the target products were separated. Results Three compounds including Enterocin (1), 5-deoxyenterocin (2) and 3-epi-5-deoxyenterocin (3) were isolated from Lactobacillus plantarum LXF-80 fermented extracts. Conclusion The experimental results prove that the addition of metyrapone affects the biosynthesis of polyketide and the intermediate compounds 2 and 3 are obtained during the formation of two enteric antibiotics.