论文部分内容阅读
对α粒子照射诱发人类11号染色体(Hchr11)基因突变的旁效应及其可能的机理进行研究。用包含单条Hchr11的人?中国仓鼠卵巢细胞杂交细胞系(AL)为靶细胞,经CD59表面抗原抗体在补体存在下筛选突变细胞克隆,测定Hchr11基因突变率;;在α粒子照射源与受照射细胞间插入网格,定比例照射细胞,观察α粒子照射的细胞对周围未受照射细胞基因突变的影响,即旁效应;;通过观察自由基清除剂二甲基亚砜(DMSO)和细胞间通讯阻断剂高丙体六六六(Lindane)对α粒子照射诱发Hchr11基因突变旁效应的抑制作用探讨其可能的机理。单纯α子照射细胞诱发的基因突变率与照射剂量存在明确的剂量效应关系;;用α粒子加网照射的实验模型,仅15%的细胞受到照射时,群体细胞的基因突变率明显高于受照射细胞的预期基因突变率,表明未受照射的细胞中也发生了基因突变;;DMSO能显著减少α粒子照射细胞诱发的基因突变,但对其诱发的基因突变旁效应无明显抑制作用;;与此相反,Lindane对单纯α粒子照射细胞诱发的基因突变无明显影响,但能显著降低α粒子照射细胞诱发的基因突变旁效应。α粒子照射细胞诱发的基因突变存在旁效应;;细胞间通讯在α粒子照射诱发细胞基因突变旁效应中起重要作用。
The side effect and possible mechanism of α particle irradiation on human chromosome 11 (Hchr11) gene mutation were studied. Human Hamster Ovary cells hybrid cell line (AL) containing a single Hchr11 was used as a target cell, and the mutant cells were screened by CD59 surface antigen antibody in the presence of complement to determine the mutation rate of Hchr11 gene. Cells were intercalated into the grid cells and the cells were irradiated in proportion to observe the effect of α-particle-irradiated cells on the gene mutation in the surrounding non-irradiated cells, that is, the side effect. By observing the effect of the free radical scavenger dimethylsulfoxide (DMSO) The inhibitory effect of Lindane, a blocker of communication block, on the side effects of Hchr11 gene mutation induced by α particle irradiation was investigated. There was a clear dose-response relationship between the gene mutation rates induced by pure α-irradiated cells and irradiation dose. The mutation rate of population cells was significantly higher than that of irradiated cells when only 15% The expected gene mutation rate of irradiated cells showed that gene mutation also occurred in unirradiated cells. DMSO could significantly reduce the gene mutation induced by α particle-irradiated cells, but had no obvious inhibitory effect on the para-side effects induced by α-particle irradiation. In contrast, Lindane had no significant effect on the gene mutation induced by pure alpha particle irradiation, but significantly reduced the side effect of gene mutation induced by alpha particle irradiation. Alpha-particle-irradiated cells induce side-effects of gene mutations ;; Intercellular communication plays an important role in the side-by-side effects of alpha particle-induced cellular gene mutations.