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制备了两种水溶性甲壳素:N-乙酰化壳聚糖(N-ACTS,DS=0.55)和6-O-羟乙基甲壳素(6-O-GC,DS=1.0),将其分别与丝素(SF)混合,制备了水溶性均相混合的复合膜;红外光谱(FT-IR)证明了均相混合的复合膜中两种天然高分子官能团结构的变化,相互之间形成了氢键结合,成为均一的混合体,表明天然高分子之间具有良好的相容性;原子间力显微镜(AFM)表面形貌观察表明:均相混合复合膜的表面比单一丝素蛋白质和多糖螺旋立体构造分布均匀表面光滑;体外细胞培养实验表明:人皮肤纤维芽细胞在两种复合膜上均生长良好,具有良好的生物相容性和医学应用前景。
Two kinds of water-soluble chitin were prepared: N-acetylated chitosan (N-ACTS, DS = 0.55) and 6-O-hydroxyethylchitin (6-O-GC, DS = 1.0) And mixed with silk fibroin (SF) to prepare a water-soluble homogeneous composite membrane; FT-IR (Infrared Spectroscopy) proved that the two natural polymer functional groups in the homogeneous mixed membrane structure changes, formed between each other Hydrogen bonding, to become a homogeneous mixture, indicating that there is a good compatibility between natural polymers; atomic force microscopy (AFM) surface morphology observation shows that: homogeneous mixed composite membrane surface than the single silk protein and polysaccharide Spiral three-dimensional structure of uniform distribution of smooth surface; in vitro cell culture experiments show that: human skin fibroblasts grow well on both composite membranes, has good biocompatibility and medical application prospects.