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目的通过复制人肝癌细胞株HepG2裸鼠皮下移植瘤模型,观察绿茶提取物表没食子儿茶素没食子酸酯(EGCG)干预对HepG2移植瘤新生血管生成的影响。方法瘤体接种复制HepG2移植瘤模型,荷瘤裸鼠20只随机分组,实验组给予EGCG溶液每日20 mg/(kg.只),腹腔注射3周,对照组给予等量灭菌注射用水3周,末次用药24 h,后处死裸鼠,剥离移植瘤。常规病理切片观察移植瘤组织结构;逆转录-聚合酶链式反应和免疫组织化学法检测移植瘤缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)mRNA及蛋白表达,并通过检测CD34表达计数瘤组织微血管密度(MVD)。结果组织病理学观察实验组移植瘤见大量坏死区,瘤体内血管数量明显少于对照组;实验组HIF-1α、VEGF mRNA及蛋白表达水平比对照组均明显下调(P<0.05),实验组MVD比对照组明显下降(P<0.05)。结论 EGCG可抑制荷瘤裸鼠HepG2移植瘤新生血管生成。
OBJECTIVE: To investigate the effect of epigallocatechin-3-gallate (EGCG) on neovascularization of HepG2 xenografts in nude mice by transfection of human hepatocellular carcinoma cell line HepG2 into nude mice. Methods The tumor-bearing HepG2 xenografts were inoculated with replicative HepG2 cells. Twenty tumor-bearing nude mice were randomly divided into experimental group and EGCG group. The experimental group was given 20 mg / (kg) EGCG daily for 3 weeks. The control group received equal volume of water for injection 3 Week, the last medication 24 h, after the death of nude mice, stripped of xenografts. The histopathology of the tumor was observed by routine pathology. The mRNA and protein expression of HIF-1α and VEGF were detected by RT-PCR and immunohistochemistry. Tumor microvessel density (MVD) was counted by detecting CD34 expression. Results Histopathological observation showed that a large number of necrotic areas were found in the experimental group and the number of blood vessels in the tumor was significantly less than that in the control group. The mRNA and protein expressions of HIF-1α and VEGF in the experimental group were significantly lower than those in the control group (P <0.05) MVD was significantly lower than the control group (P <0.05). Conclusion EGCG can inhibit angiogenesis in HepG2 transplanted tumor in nude mice.