论文部分内容阅读
目的 观察p16基因在胃癌中的表达及其临床应用价值。方法 应用SP免疫组化及原位杂交技术检测人胃癌中 p16蛋白及其mRNA的表达。 结果 p16蛋白在胃癌中的表达阳性率为 6 5 .88% (5 6 / 85 ) ,阴性表达者浸润较深、分化较差 ,术后生存期较短 (P <0 .0 5 ) ;p16mRNA在胃癌中的表达阳性率为 47.37% (18/ 38)。 结论 p16基因与胃癌发生、进展有关 ,原位检测其表达有助于对胃癌生物学行为的了解和对患者预后的判断
Objective To observe the expression of p16 gene in gastric cancer and its clinical value. Methods SP immunohistochemistry and in situ hybridization were used to detect the expression of p16 protein and its mRNA in human gastric cancer. Results The positive expression rate of p16 protein in gastric cancer was 65.88% (56/85). The negative expression of p16 protein was significantly deeper in infiltration, poorly differentiated and shorter survival time (P <0.05). The positive rate of p16 mRNA The positive rate in gastric cancer was 47.37% (18/38). Conclusion The p16 gene is related to the occurrence and progression of gastric cancer. Detecting the expression of p16 gene in situ helps to understand the biological behavior of gastric cancer and to judge the prognosis of patients