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目的优化穿山龙总皂苷的提取工艺。方法采取超声波辅助纤维素酶法提取穿山龙总皂苷;以单因素实验结果为基础,利用响应面设计系统分析且优化影响总皂苷含量的主要因素,从而建立影响因素与对应的响应值之间的数学关系模型,得到最优提取条件。结果最佳提取工艺条件为pH值6.0,水浴温度31.3℃,乙醇浓度44.21%,总皂苷得率为0.421%。结论优化所得工艺操作方便、效率高,适合工业化大生产。
Objective To optimize the extraction process of total saponins from Chrysanthemum morifolium. Methods Ultrasound-assisted cellulase method was used to extract total saponin from Asparagus officinalis. Based on the single-factor experimental results, the main factors influencing the total saponin content were analyzed and optimized by using response surface methodology to establish the relationship between the response factors and the corresponding factors Relationship model, the optimal extraction conditions. Results The optimal extraction conditions were pH 6.0, water bath temperature 31.3 ℃, ethanol concentration 44.21% and total saponin yield 0.421%. Conclusion The optimized process is easy to operate with high efficiency and suitable for industrialized large-scale production.