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选用3～6月龄健康家兔18只,分为对照组、模型组、全肺灌洗组3个组,每组6只;分别对模型组、全肺灌洗组家兔按体表面积予以500 mg/m2的百草枯生理盐水溶液灌胃,建立百草枯致肺纤维化模型,对照组以相等剂量生理盐水灌胃;分别于染毒前及染毒后14 d、21 d、28 d经耳中央动脉采集各组动物动脉血,行血气分析,记录氧分压值,比较各组血氧分压差异。灌洗组于染毒后24 h和48 h分别行左肺和右肺灌洗。于染毒后28 d动脉血气分析完成后,分别处死各组动物,留取右肺中叶标本,固定,制作HE染色、Masson三色染色病理切片,按Szapiel等的半定量方法判断肺纤维化程度,比较各组间肺纤维化程度。染毒前各组动物血气分析氧分压值差异无统计学意义(P>0.05);染毒后14 d、21 d、28 d氧分压值差异具有统计学意义(P<0.05),模型组与全肺灌洗组明显低于对照组,全肺灌洗组明显优于模型组(P<0.05)。染毒后28 d各组动物肺纤维化程度差异具有统计学意义;模型组与全肺灌洗组肺纤维化程度明显高于对照组,差异具有统计学意义(P<0.05);模型组肺纤维化程度明显高于全肺灌洗组,差异具有统计学意义(P<0.05)。提示全肺灌洗对百草枯所致肺纤维化具有一定治疗效果。
Eighteen healthy 3- and 6-month-old rabbits were divided into three groups: control group, model group and whole lung lavage group, with 6 rats in each group. According to the body surface area of the model group and the whole lung lavage group, 500 mg / m2 paraquat physiological saline solution was orally administered to establish a model of paraquat-induced pulmonary fibrosis in the control group with the same dose of normal saline; before and after the exposure, and on the 14th, 21st and 28th days after exposure Ear central artery of each group of animals were collected arterial blood gas analysis, recording oxygen partial pressure values, compared the difference between the partial pressure of blood oxygen. Lavage group were left lung and right lung lavage at 24 h and 48 h after exposure. After the arterial blood gas analysis was completed on the 28th day after exposure, the animals in each group were sacrificed and the right middle lobe samples were collected and fixed. HE staining and Masson trichrome staining were performed. The degree of pulmonary fibrosis was determined according to Szapiel’s semi-quantitative method , Compare the degree of pulmonary fibrosis among the groups. There was no significant difference in the partial pressure of oxygen between the two groups before exposure (P> 0.05). The differences of oxygen partial pressure at 14 d, 21 d and 28 d after exposure were statistically significant (P <0.05) Group and the whole lung lavage group was significantly lower than the control group, the whole lung lavage group was significantly better than the model group (P <0.05). The degree of pulmonary fibrosis in each group was statistically significant at 28 days after exposure. The degree of pulmonary fibrosis in model group and whole lung lavage group was significantly higher than that in control group (P <0.05) Fibrosis was significantly higher than the whole lung lavage group, the difference was statistically significant (P <0.05). Tip of the whole lung lavage paraquat-induced pulmonary fibrosis have a certain therapeutic effect.